The LI-8250 Multiplexer has 8 ports. Each multiplexer port can connect to either a chamber or an 8250-01 Extension Manifold. You can have a maximum of 4 extension manifolds connected to the LI-8250 Multiplexer.

Each extension manifold also has 8 ports that can connect to chambers. Since the extension manifold uses 1 multiplexer port, each extension manifold gains you up to 7 more chambers.

With 4 extension manifolds, you can have 32 chambers on extension manifold ports and another 4 chambers on the remaining multiplexer ports.